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Immunoglobulin and T cell receptor repertoire changes induced by a prototype vaccine against Chagas disease in naïve rhesus macaques.
Dumonteil, Eric; Tu, Weihong; Desale, Hans; Goff, Kelly; Marx, Preston; Ortega-Lopez, Jaime; Herrera, Claudia.
Afiliação
  • Dumonteil E; Department of Tropical Medicine and Infectious Disease, School of Public Health and Tropical Medicine, and Vector-Borne and Infectious Disease Research Center, Tulane University, 1440 Canal St, New Orleans, Louisiana, 70112, USA. edumonte@tulane.edu.
  • Tu W; Department of Tropical Medicine and Infectious Disease, School of Public Health and Tropical Medicine, and Vector-Borne and Infectious Disease Research Center, Tulane University, 1440 Canal St, New Orleans, Louisiana, 70112, USA.
  • Desale H; Department of Tropical Medicine and Infectious Disease, School of Public Health and Tropical Medicine, and Vector-Borne and Infectious Disease Research Center, Tulane University, 1440 Canal St, New Orleans, Louisiana, 70112, USA.
  • Goff K; Division of Microbiology, Tulane National Primate Research Center, Tulane University, Covington, LA, USA.
  • Marx P; Department of Tropical Medicine and Infectious Disease, School of Public Health and Tropical Medicine, and Vector-Borne and Infectious Disease Research Center, Tulane University, 1440 Canal St, New Orleans, Louisiana, 70112, USA.
  • Ortega-Lopez J; Division of Microbiology, Tulane National Primate Research Center, Tulane University, Covington, LA, USA.
  • Herrera C; Departamento de Biotecnología y Bioingeniería, Centro de Investigación y Estudios Avanzados del Instituto Politécnico Nacional, Ciudad de Mexico, México.
J Biomed Sci ; 31(1): 58, 2024 Jun 01.
Article em En | MEDLINE | ID: mdl-38824576
ABSTRACT

BACKGROUND:

A vaccine against Trypanosoma cruzi, the agent of Chagas disease, would be an excellent additional tool for disease control. A recombinant vaccine based on Tc24 and TSA1 parasite antigens was found to be safe and immunogenic in naïve macaques.

METHODS:

We used RNA-sequencing and performed a transcriptomic analysis of PBMC responses to vaccination of naïve macaques after each vaccine dose, to shed light on the immunogenicity of this vaccine and guide the optimization of doses and formulation. We identified differentially expressed genes and pathways and characterized immunoglobulin and T cell receptor repertoires.

RESULTS:

RNA-sequencing analysis indicated a clear transcriptomic response of PBMCs after three vaccine doses, with the up-regulation of several immune cell activation pathways and a broad non-polarized immune profile. Analysis of the IgG repertoire showed that it had a rapid turnover with novel IgGs produced following each vaccine dose, while the TCR repertoire presented several persisting clones that were expanded after each vaccine dose.

CONCLUSIONS:

These data suggest that three vaccine doses may be needed for optimum immunogenicity and support the further evaluation of the protective efficacy of this vaccine.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Receptores de Antígenos de Linfócitos T / Vacinas Protozoárias / Doença de Chagas / Macaca mulatta Limite: Animals Idioma: En Revista: J Biomed Sci Assunto da revista: MEDICINA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Receptores de Antígenos de Linfócitos T / Vacinas Protozoárias / Doença de Chagas / Macaca mulatta Limite: Animals Idioma: En Revista: J Biomed Sci Assunto da revista: MEDICINA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Estados Unidos