Towards Development of the 4C-Based Method Detecting Interactions of Plasmid DNA with Host Genome.
Biochemistry (Mosc)
; 89(4): 653-662, 2024 Apr.
Article
em En
| MEDLINE
| ID: mdl-38831502
ABSTRACT
Chromosome conformation capture techniques have revolutionized our understanding of chromatin architecture and dynamics at the genome-wide scale. In recent years, these methods have been applied to a diverse array of species, revealing fundamental principles of chromosomal organization. However, structural organization of the extrachromosomal entities, like viral genomes or plasmids, and their interactions with the host genome, remain relatively underexplored. In this work, we introduce an enhanced 4C-protocol tailored for probing plasmid DNA interactions. We design specific plasmid vector and optimize protocol to allow high detection rate of contacts between the plasmid and host DNA.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plasmídeos
Idioma:
En
Revista:
Biochemistry (Mosc)
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Federação Russa