Your browser doesn't support javascript.
loading
Concurrent D-loop cleavage by Mus81 and Yen1 yields half-crossover precursors.
Carreira, Raquel; Lama-Diaz, Tomas; Crugeiras, Maria; Aguado, F Javier; Sebesta, Marek; Krejci, Lumir; Blanco, Miguel G.
Afiliação
  • Carreira R; Department of Biochemistry and Molecular Biology, CIMUS, Universidade de Santiago de Compostela-Instituto de Investigación Sanitaria (IDIS), Santiago de Compostela, A Coruña 15782, Spain.
  • Lama-Diaz T; Department of Biochemistry and Molecular Biology, CIMUS, Universidade de Santiago de Compostela-Instituto de Investigación Sanitaria (IDIS), Santiago de Compostela, A Coruña 15782, Spain.
  • Crugeiras M; Department of Biochemistry and Molecular Biology, CIMUS, Universidade de Santiago de Compostela-Instituto de Investigación Sanitaria (IDIS), Santiago de Compostela, A Coruña 15782, Spain.
  • Aguado FJ; Department of Biochemistry and Molecular Biology, CIMUS, Universidade de Santiago de Compostela-Instituto de Investigación Sanitaria (IDIS), Santiago de Compostela, A Coruña 15782, Spain.
  • Sebesta M; Department of Biology and National Centre for Biomolecular Research, Masaryk University, Brno 62500, Czech Republic.
  • Krejci L; Department of Biology and National Centre for Biomolecular Research, Masaryk University, Brno 62500, Czech Republic.
  • Blanco MG; Department of Biochemistry and Molecular Biology, CIMUS, Universidade de Santiago de Compostela-Instituto de Investigación Sanitaria (IDIS), Santiago de Compostela, A Coruña 15782, Spain.
Nucleic Acids Res ; 52(12): 7012-7030, 2024 Jul 08.
Article em En | MEDLINE | ID: mdl-38832625
ABSTRACT
Homologous recombination involves the formation of branched DNA molecules that may interfere with chromosome segregation. To resolve these persistent joint molecules, cells rely on the activation of structure-selective endonucleases (SSEs) during the late stages of the cell cycle. However, the premature activation of SSEs compromises genome integrity, due to untimely processing of replication and/or recombination intermediates. Here, we used a biochemical approach to show that the budding yeast SSEs Mus81 and Yen1 possess the ability to cleave the central recombination intermediate known as the displacement loop or D-loop. Moreover, we demonstrate that, consistently with previous genetic data, the simultaneous action of Mus81 and Yen1, followed by ligation, is sufficient to recreate the formation of a half-crossover precursor in vitro. Our results provide not only mechanistic explanation for the formation of a half-crossover, but also highlight the critical importance for precise regulation of these SSEs to prevent chromosomal rearrangements.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Troca Genética / Proteínas de Saccharomyces cerevisiae / Proteínas de Ligação a DNA / Endonucleases Idioma: En Revista: Nucleic Acids Res Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Espanha

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Troca Genética / Proteínas de Saccharomyces cerevisiae / Proteínas de Ligação a DNA / Endonucleases Idioma: En Revista: Nucleic Acids Res Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Espanha