Qualitative real-time RT-PCR assay for nOPV2 poliovirus detection.
J Virol Methods
; 329: 114984, 2024 Sep.
Article
em En
| MEDLINE
| ID: mdl-38885908
ABSTRACT
Based on the success of the Sabin2-based vaccine, a next-generation nOPV2 poliovirus vaccine has been developed. For epidemic monitoring and conducting epidemiological investigations, it is necessary to have a diagnostic assay with the ability to differentiate this variant from others. Here we describe such a real-time RT-PCR assay. The region with the cre insertion in the 5'-UTR was chosen as the target, and the limit of detection was 103 copies/mL (2.5×103 copies/mL using Probit analysis) determined using armored RNA particles. Sensitivity and specificity were 86.28 - 100â¯% and 76.84 - 100â¯%, respectively (with 95â¯% CI). Thus, this method can be effectively used when it is necessary to make a differential diagnosis of poliovirus strains.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Poliomielite
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RNA Viral
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Sensibilidade e Especificidade
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Reação em Cadeia da Polimerase Via Transcriptase Reversa
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Poliovirus
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Reação em Cadeia da Polimerase em Tempo Real
Limite:
Humans
Idioma:
En
Revista:
J Virol Methods
Ano de publicação:
2024
Tipo de documento:
Article