MEX3A promotes colorectal cancer migration, invasion and EMT via regulating the Wnt/ß-catenin signaling pathway.
J Cancer Res Clin Oncol
; 150(6): 319, 2024 Jun 25.
Article
em En
| MEDLINE
| ID: mdl-38914858
ABSTRACT
BACKGROUND:
Mex-3 RNA binding family members are well-established to be important in cancer development and progression. However, the functions of Mex-3 RNA binding family member A (MEX3A) in colorectal cancer (CRC) metastasis remain poorly understood. In this study, we aim to reveal the function and the mechanism of MEX3A in promoting CRC metastasis.METHODS:
We used multiple databases including TCGA database, UALCAN, LinkedOmics, CancerSEA, GeneMANIA and STRING database to investigate the expression, the functions and underlying molecular mechanism of MEX3A in CRC. Multiple experimental methods were adapted to determine the study, including real-time PCR (qPCR), immunohistochemistry (IHC), western blot (WB), transfection, transwell migration and invasion assays, immunofluorescence (IF).RESULTS:
We found that MEX3A was significantly upregulated and correlated to tumor stage and lymph nodal metastasis in CRC through bioinformatics analysis and tissue immunohistochemistry (IHC). The higher expression of MEX3A in CRC correlated with poor recurrence-free survival (RFS) and overall survival (OS). In vitro studies showed that knockdown of MEX3A suppressed EMT transition, invasion and metastasis of CRC cells. Mechanistically, we revealed that MEX3A promotes epithelial-mesenchymal transition (EMT), invasion and metastasis of CRC cells by upregulating the Wnt/ß-catenin signaling pathway.CONCLUSION:
In conclusion, our study reveals that MEX3A promotes CRC migration, invasion and EMT via regulating the Wnt/ß-catenin signaling pathway and could be a novel therapeutic target for this patient population.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neoplasias Colorretais
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Movimento Celular
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Proteínas de Ligação a RNA
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Transição Epitelial-Mesenquimal
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Via de Sinalização Wnt
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Invasividade Neoplásica
Limite:
Female
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Humans
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Male
Idioma:
En
Revista:
J Cancer Res Clin Oncol
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
China