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Generation of heterozygous (MCRIi031-A-1) and homozygous (MCRIi031-A-2) SOX9 knockout human iPSC lines.
Pachernegg, Svenja; Robevska, Gorjana; G A Ferreira, Lucas; van den Bergen, Jocelyn A; Vlahos, Katerina; Howden, Sara E; Sinclair, Andrew H; Ayers, Katie L.
Afiliação
  • Pachernegg S; Reproductive Development, Murdoch Children's Research Institute, Melbourne, Australia; Department of Paediatrics, The University of Melbourne, Melbourne, Australia.
  • Robevska G; Reproductive Development, Murdoch Children's Research Institute, Melbourne, Australia.
  • G A Ferreira L; Reproductive Development, Murdoch Children's Research Institute, Melbourne, Australia; Laboratory of Molecular and Translational Endocrinology, Universidade Federal de Sao Paulo, Sao Paulo, Brazil.
  • van den Bergen JA; Reproductive Development, Murdoch Children's Research Institute, Melbourne, Australia.
  • Vlahos K; iPSC Derivation and Gene Editing Facility, Murdoch Children's Research Institute, Melbourne, Australia.
  • Howden SE; Department of Paediatrics, The University of Melbourne, Melbourne, Australia; iPSC Derivation and Gene Editing Facility, Murdoch Children's Research Institute, Melbourne, Australia; Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Murdoch Children's Research Institute, Melbourne, Austr
  • Sinclair AH; Reproductive Development, Murdoch Children's Research Institute, Melbourne, Australia; Department of Paediatrics, The University of Melbourne, Melbourne, Australia.
  • Ayers KL; Reproductive Development, Murdoch Children's Research Institute, Melbourne, Australia; Department of Paediatrics, The University of Melbourne, Melbourne, Australia. Electronic address: katie.ayers@mcri.edu.au.
Stem Cell Res ; 79: 103484, 2024 Sep.
Article em En | MEDLINE | ID: mdl-38924973
ABSTRACT
The transcription factor SOX9 plays a critical role in several embryonic developmental processes such as gonadogenesis, chrondrogenesis, and cardiac development. We generated heterozygous (MCRIi031-A-1) and homozygous (MCRIi031-A-2) SOX9 knockout induced pluripotent stem cell (iPSC) lines from human fibroblasts using a one-step protocol for CRISPR/Cas9 gene-editing and episomal-based reprogramming. Both iPSC lines exhibit a normal karyotype and morphology, express pluripotency markers, and have the capacity to differentiate into the three embryonic germ layers. These cell lines will allow us to further explore the role of SOX9 in critical developmental processes.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fatores de Transcrição SOX9 / Células-Tronco Pluripotentes Induzidas / Heterozigoto / Homozigoto Limite: Humans Idioma: En Revista: Stem Cell Res Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Austrália
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fatores de Transcrição SOX9 / Células-Tronco Pluripotentes Induzidas / Heterozigoto / Homozigoto Limite: Humans Idioma: En Revista: Stem Cell Res Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Austrália