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Haloperidol, Olanzapine, and Risperidone Induce Morphological Changes in an In Vitro Model of Human Hippocampal Neurogenesis.
Jezsó, Bálint; Kálmán, Sára; Farkas, Kiara Gitta; Hathy, Edit; Vincze, Katalin; Kovács-Schoblocher, Dzsenifer; Lilienberg, Julianna; Tordai, Csongor; Nemoda, Zsófia; Homolya, László; Apáti, Ágota; Réthelyi, János M.
Afiliação
  • Jezsó B; Institute of Molecular Life Sciences, HUN-REN RCNS, Magyar tudósok körútja 2., H-1117 Budapest, Hungary.
  • Kálmán S; Doctoral School of Biology, Institute of Biology, Eötvös Loránd University, Pázmány Péter sétány 1/c., H-1117 Budapest, Hungary.
  • Farkas KG; ELTE-MTA "Momentum" Motor Enzymology Research Group, Department of Biochemistry, Eötvös Loránd University, Pázmány Péter sétány 1/c., H-1117 Budapest, Hungary.
  • Hathy E; Albert Szent-Györgyi Health Centre, Department of Psychiatry, University of Szeged, Szentháromság utca 5., H-6722 Szeged, Hungary.
  • Vincze K; Institute of Molecular Life Sciences, HUN-REN RCNS, Magyar tudósok körútja 2., H-1117 Budapest, Hungary.
  • Kovács-Schoblocher D; Institute of Molecular Life Sciences, HUN-REN RCNS, Magyar tudósok körútja 2., H-1117 Budapest, Hungary.
  • Lilienberg J; Department of Psychiatry and Psychotherapy, Semmelweis University, Balassa utca 6., H-1083 Budapest, Hungary.
  • Tordai C; Institute of Molecular Life Sciences, HUN-REN RCNS, Magyar tudósok körútja 2., H-1117 Budapest, Hungary.
  • Nemoda Z; Department of Psychiatry and Psychotherapy, Semmelweis University, Balassa utca 6., H-1083 Budapest, Hungary.
  • Homolya L; Institute of Molecular Life Sciences, HUN-REN RCNS, Magyar tudósok körútja 2., H-1117 Budapest, Hungary.
  • Apáti Á; Institute of Molecular Life Sciences, HUN-REN RCNS, Magyar tudósok körútja 2., H-1117 Budapest, Hungary.
  • Réthelyi JM; Institute of Molecular Life Sciences, HUN-REN RCNS, Magyar tudósok körútja 2., H-1117 Budapest, Hungary.
Biomolecules ; 14(6)2024 Jun 13.
Article em En | MEDLINE | ID: mdl-38927091
ABSTRACT

BACKGROUND:

Induced pluripotent stem cell (iPSC) based neuronal differentiation is valuable for studying neuropsychiatric disorders and pharmacological mechanisms at the cellular level. We aimed to examine the effects of typical and atypical antipsychotics on human iPSC-derived neural progenitor cells (NPCs).

METHODS:

Proliferation and neurite outgrowth were measured by live cell imaging, and gene expression levels related to neuronal identity were analyzed by RT-QPCR and immunocytochemistry during differentiation into hippocampal dentate gyrus granule cells following treatment of low- and high-dose antipsychotics (haloperidol, olanzapine, and risperidone).

RESULTS:

Antipsychotics did not modify the growth properties of NPCs after 3 days of treatment. However, the characteristics of neurite outgrowth changed significantly in response to haloperidol and olanzapine. After three weeks of differentiation, mRNA expression levels of the selected neuronal markers increased (except for MAP2), while antipsychotics caused only subtle changes. Additionally, we found no changes in MAP2 or GFAP protein expression levels as a result of antipsychotic treatment.

CONCLUSIONS:

Altogether, antipsychotic medications promoted neurogenesis in vitro by influencing neurite outgrowth rather than changing cell survival or gene expression. This study provides insights into the effects of antipsychotics on neuronal differentiation and highlights the importance of considering neurite outgrowth as a potential target of action.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Antipsicóticos / Diferenciação Celular / Risperidona / Neurogênese / Células-Tronco Pluripotentes Induzidas / Células-Tronco Neurais / Olanzapina / Haloperidol / Hipocampo Limite: Humans Idioma: En Revista: Biomolecules Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Hungria

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Antipsicóticos / Diferenciação Celular / Risperidona / Neurogênese / Células-Tronco Pluripotentes Induzidas / Células-Tronco Neurais / Olanzapina / Haloperidol / Hipocampo Limite: Humans Idioma: En Revista: Biomolecules Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Hungria