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Ehmt2 inactivation in pancreatic epithelial cells shapes the transcriptional landscape and inflammation response of the whole pancreas.
Pollin, Gareth; Mathison, Angela J; de Assuncao, Thiago M; Thomas, Anju; Zeighami, Atefeh; Salmonson, Ann; Liu, Hongfei; Urrutia, Guillermo; Vankayala, Pallavi; Pandol, Stephen J; Hong, Johnny C; Zimmermann, Michael T; Iovanna, Juan; Jin, Victor X; Urrutia, Raul; Lomberk, Gwen.
Afiliação
  • Pollin G; Linda T. and John A. Mellowes Center for Genomic Sciences and Precision Medicine, Medical College of Wisconsin, Milwaukee, WI, United States.
  • Mathison AJ; Division of Research, Department of Surgery, Medical College of Wisconsin, Milwaukee, WI, United States.
  • de Assuncao TM; Linda T. and John A. Mellowes Center for Genomic Sciences and Precision Medicine, Medical College of Wisconsin, Milwaukee, WI, United States.
  • Thomas A; Division of Research, Department of Surgery, Medical College of Wisconsin, Milwaukee, WI, United States.
  • Zeighami A; Linda T. and John A. Mellowes Center for Genomic Sciences and Precision Medicine, Medical College of Wisconsin, Milwaukee, WI, United States.
  • Salmonson A; Division of Research, Department of Surgery, Medical College of Wisconsin, Milwaukee, WI, United States.
  • Liu H; Linda T. and John A. Mellowes Center for Genomic Sciences and Precision Medicine, Medical College of Wisconsin, Milwaukee, WI, United States.
  • Urrutia G; Division of Research, Department of Surgery, Medical College of Wisconsin, Milwaukee, WI, United States.
  • Vankayala P; Linda T. and John A. Mellowes Center for Genomic Sciences and Precision Medicine, Medical College of Wisconsin, Milwaukee, WI, United States.
  • Pandol SJ; Division of Research, Department of Surgery, Medical College of Wisconsin, Milwaukee, WI, United States.
  • Hong JC; Linda T. and John A. Mellowes Center for Genomic Sciences and Precision Medicine, Medical College of Wisconsin, Milwaukee, WI, United States.
  • Zimmermann MT; Division of Research, Department of Surgery, Medical College of Wisconsin, Milwaukee, WI, United States.
  • Iovanna J; Linda T. and John A. Mellowes Center for Genomic Sciences and Precision Medicine, Medical College of Wisconsin, Milwaukee, WI, United States.
  • Jin VX; Department of Medicine, Cedars-Sinai Medical Center, Los Angeles, CA, United States.
  • Urrutia R; Division of Transplantation, Department of Surgery, College of Medicine, Pennsylvania State University, Hershey, PA, United States.
  • Lomberk G; Linda T. and John A. Mellowes Center for Genomic Sciences and Precision Medicine, Medical College of Wisconsin, Milwaukee, WI, United States.
Front Genet ; 15: 1412767, 2024.
Article em En | MEDLINE | ID: mdl-38948355
ABSTRACT

Introduction:

The Euchromatic Histone Methyl Transferase Protein 2 (EHMT2), also known as G9a, deposits transcriptionally repressive chromatin marks that play pivotal roles in the maturation and homeostasis of multiple organs. Recently, we have shown that Ehmt2 inactivation in the mouse pancreas alters growth and immune gene expression networks, antagonizing Kras-mediated pancreatic cancer initiation and promotion. Here, we elucidate the essential role of Ehmt2 in maintaining a transcriptional landscape that protects organs from inflammation.

Methods:

Comparative RNA-seq studies between normal postnatal and young adult pancreatic tissue from Ehmt2 conditional knockout animals (Ehmt2 fl/fl ) targeted to the exocrine pancreatic epithelial cells (Pdx1-Cre and P48 Cre/+ ), reveal alterations in gene expression networks in the whole organ related to injury-inflammation-repair, suggesting an increased predisposition to damage. Thus, we induced an inflammation repair response in the Ehmt2 fl/fl pancreas and used a data science-based approach to integrate RNA-seq-derived pathways and networks, deconvolution digital cytology, and spatial transcriptomics. We also analyzed the tissue response to damage at the morphological, biochemical, and molecular pathology levels. Results and

discussion:

The Ehmt2 fl/fl pancreas displays an enhanced injury-inflammation-repair response, offering insights into fundamental molecular and cellular mechanisms involved in this process. More importantly, these data show that conditional Ehmt2 inactivation in exocrine cells reprograms the local environment to recruit mesenchymal and immunological cells needed to mount an increased inflammatory response. Mechanistically, this response is an enhanced injury-inflammation-repair reaction with a small contribution of specific Ehmt2-regulated transcripts. Thus, this new knowledge extends the mechanisms underlying the role of the Ehmt2-mediated pathway in suppressing pancreatic cancer initiation and modulating inflammatory pancreatic diseases.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Front Genet Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Front Genet Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Estados Unidos