Acoustic-holography-patterned primary hepatocytes possess liver functions.
Biomaterials
; 311: 122691, 2024 Dec.
Article
em En
| MEDLINE
| ID: mdl-38996673
ABSTRACT
Acoustic holography (AH), a promising approach for cell patterning, emerges as a powerful tool for constructing novel invitro 3D models that mimic organs and cancers features. However, understanding changes in cell function post-AH remains limited. Furthermore, replicating complex physiological and pathological processes solely with cell lines proves challenging. Here, we employed acoustical holographic lattice to assemble primary hepatocytes directly isolated from mice into a cell cluster matrix to construct a liver-shaped tissue sample. For the first time, we evaluated the liver functions of AH-patterned primary hepatocytes. The patterned model exhibited large numbers of self-assembled spheroids and superior multifarious core hepatocyte functions compared to cells in 2D and traditional 3D culture models. AH offers a robust protocol for long-term in vitro culture of primary cells, underscoring its potential for future applications in disease pathogenesis research, drug testing, and organ replacement therapy.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Holografia
/
Hepatócitos
/
Fígado
Limite:
Animals
Idioma:
En
Revista:
Biomaterials
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
China