Electrophoretic characterization of active renin from human kidney and inactive renin from a human chorionic cell culture.
Mol Cell Endocrinol
; 42(2): 175-83, 1985 Sep.
Article
em En
| MEDLINE
| ID: mdl-3905453
ABSTRACT
Enzymatically inactive human renin from chorionic cells in culture is significantly distinct in polyacrylamide gel electrophoresis (pH 8.17, 0 degree C) from active human kidney renin. The inactive renin is larger and more basic than the active renin; their molecular weights derived from gel electrophoretic retardation coefficients relate as 47.5/35.3 kDa, their valences (net protons/molecule) as 2.14/1.85. In gel electrofocusing conducted in a mixture of simple buffers, both inactive and active renins exhibit 2 components at the steady-state. The molecular size and basicity of inactive renin are consistent with the hypothesis that it may be a precursor (prorenin), although the possibility that it is an inhibitor complex cannot be ruled out.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Córion
/
Renina
/
Rim
Limite:
Humans
Idioma:
En
Revista:
Mol Cell Endocrinol
Ano de publicação:
1985
Tipo de documento:
Article