Suppressive effect of nonviable Mycoplasma hyopneumoniae on phytohemagglutinin-induced transformation of swine lymphocytes.
Am J Vet Res
; 46(11): 2366-8, 1985 Nov.
Article
em En
| MEDLINE
| ID: mdl-4073648
ABSTRACT
The effect of nonviable Mycoplasma hyopneumoniae on transformation of swine peripheral blood lymphocytes by mitogen was investigated. Lymphocyte transformation was evaluated as incorporation of [3H]-thymidine, using a microculture system. Mycoplasma hyopneumoniae was grown in Friis medium, inactivated with sodium azide, and washed with phosphate-buffered saline solution. Four strains of M hyopneumoniae, strain J, strain 11, and 2 low-passage isolates (1361A, 1375C), were found to suppress phytohemagglutinin-induced lymphocyte transformation. Mycoplasma hyopneumoniae strains J, 11, and 1361A reduced lymphocyte transformation by about 50%, whereas strain 1375C reduced lymphocyte transformation by 98.7%. The suppressive effect was abrogated by heating M hyopneumoniae at 60 C or at higher temperatures for 30 minutes. Sonication of the heated M hyopneumoniae cells partially restored the suppressive effect.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fito-Hemaglutininas
/
Suínos
/
Ativação Linfocitária
/
Mycoplasma
Limite:
Animals
Idioma:
En
Revista:
Am J Vet Res
Ano de publicação:
1985
Tipo de documento:
Article