The localization of spectrin on the inner surface of human red blood cell membranes by ferritin-conjugated antibodies.
J Cell Biol
; 51(1): 265-72, 1971 Oct.
Article
em En
| MEDLINE
| ID: mdl-5000071
ABSTRACT
Spectrin, a major protein constituent of mammalian red blood cell membrane preparations, has been localized on the inner surface of human red blood cell membranes by techniques that utilized specific ferritin-conjugated antibodies and fixation of membranes shortly after hemolysis so as to allow penetration of the ferritin-antibody labels. The labeling of spectrin was shown to be specific by the following criteria. (a) Nonhomologous ferritin-conjugated antibodies did not specifically bind to either membrane surface. (b) Blocking the membrane-bound spectrin with excess unconjugated antispectrin antibodies prevented ferritin-antibody labeling. (c) Removal of spectrin by treating the membrane preparation with a low ionic strength buffer containing ethylenediaminetetraacetate and beta-mercaptoethanol prevented labeling by specific ferritin-conjugated antibodies.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Sanguíneas
/
Membrana Celular
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Eritrócitos
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Ferritinas
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Anticorpos
Limite:
Animals
/
Humans
Idioma:
En
Revista:
J Cell Biol
Ano de publicação:
1971
Tipo de documento:
Article