Antigen recognition and antibody specificity. Carrier specificity and genetic control of anti-dinitrophenyl-oligolysine antibody.
J Exp Med
; 133(6): 1199-218, 1971 Jun 01.
Article
em En
| MEDLINE
| ID: mdl-5576332
ABSTRACT
The exact specifiicity of anti-DNP antibody produced by Hartley guinea pigs immunized with a series of defined alpha,DNP and epsilon,DNP-oligolysines was studied by fluorescence quenching. All responder animals made anti-DNP antibody which recognized the precise chain length, +/- 1 lysyl residue, of the DNP-oligolysines used to induce the immune response as measured by an increase in binding energy (-DeltaF degrees ) for that antigen. The ability of the immune system to detect the smallest possible change in oligolysine chain length suggests that the anti-hapten antibody-forming cell possesses a highly specific recognition system for carrier conformation. When DNP-oligolysines are incorporated in an adjuvant containing M. tuberculosis H37Rv, both responder and nonresponder produce anti-DNP antibody, but only the responder develops delayed skin sensitivity. In addition to their failure to develop delayed hypersensitivity, nonresponders produced anti-DNP oligolysine antibody which did not show the increase in -DeltaF degrees for the immunizing antigen characteristic of responder antibody. These observations support a local environment hypothesis for antigen recognition at the level of the anti-hapten antibody-forming cell and suggest that the polylysine gene exerts its control at the same cell.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Dinitrofenóis
/
Genes Dominantes
/
Anticorpos
/
Formação de Anticorpos
/
Antígenos
Limite:
Animals
Idioma:
En
Revista:
J Exp Med
Ano de publicação:
1971
Tipo de documento:
Article