[Metabolite isolation for mass spectrometrical determination of new drugs (author's transl)]. / Isolierung von Metaboliten neuer Arzneimittel zur massenspektrometrischen Strukturaufklärung.
Arzneimittelforschung
; 28(11a): 1951-60, 1978.
Article
em De
| MEDLINE
| ID: mdl-582524
ABSTRACT
Only very minute concentrations of metabolites of highly effective and consequently low dosed drugs can be detected in urine. In order to identify the structure of these compounds they must be concentrated by a factor up to 110(6). Isolation of metabolites, especially when present as conjugates, becomes more complicated the greater the polarity. In this paper some methods for isolation are presented using column- and thin-layer chromatography as well as extraction and dialysis, finally their practical application and their most effective combination are described. Priority must be given to solving two problems firstly, a rough pre-purification is necessary to eliminate physiological components from the urine such as salts, urea, etc., and secondly, the mixture of metabolites has to be separated into single metabolites. Examples are given to demonstrate the practicability of these methods for the isolation and structural identification of metabolites belonging to varying structural classes.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Preparações Farmacêuticas
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
De
Revista:
Arzneimittelforschung
Ano de publicação:
1978
Tipo de documento:
Article