Association of phenotypic reversion of transformed cells induced by interferon with morphological and biochemical changes in the cytoskeleton.
Cancer Res
; 41(10): 4174-84, 1981 Oct.
Article
em En
| MEDLINE
| ID: mdl-6169435
ABSTRACT
The distribution of microfilament bundles, intermediate filaments, microtubules, vinculin plaques, and extracellular fibronectin matrices was examined by indirect immunofluorescence in X-ray-transformed C3H/10T1/2 cells. The transformed phenotype correlated with a lack of actin cables and vinculin-containing plaques. Prolonged treatment of these transformed cells with interferon resulted in a reversion of the transformed phenotype which was associated with the reappearance of extensive microfilament bundles together with vinculin-containing plaques. Both interferon-treated and untreated transformed cells displayed a similar distribution of mitochondria, intermediate filaments, microtubules, and fibronectin. Significant qualitative and quantitative differences between the two types of cells were found in the proteins associated with the cytoskeletal framework by two-dimensional gel analysis.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Citoesqueleto
/
Transformação Celular Neoplásica
Tipo de estudo:
Qualitative_research
/
Risk_factors_studies
Limite:
Animals
Idioma:
En
Revista:
Cancer Res
Ano de publicação:
1981
Tipo de documento:
Article