Polyglutamyl peptides: a new class of inhibitors of type-2 casein kinases.
FEBS Lett
; 162(2): 235-8, 1983 Oct 17.
Article
em En
| MEDLINE
| ID: mdl-6195015
ABSTRACT
Casein kinase-TS (Ck-TS), a type-2 casein kinase purified from rat liver cytosol which phosphorylates seryl and threonyl residues N-terminal to acidic clusters, is specifically inhibited by polyglutamyl peptides which are ineffective both on type-1 casein kinase and on cAMP-dependent protein kinase. The inhibition is competitive toward the protein substrate and non-competitive toward ATP. Among the polyglutamates tested (Glu)70 is the most effective (Ki 0.11 microM). (Glu)10 and (Glu)5 are also inhibitors, though less powerful than (Glu)70, while (Glu)3, (Glu)2 and free glutamic acid up to 5 mM are ineffective. These results disclose the possibility that naturally occurring polypeptides containing long stretches of acidic residues may act as physiological inhibitors of type-2 casein kinases.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Ácido Poliglutâmico
/
Inibidores de Proteínas Quinases
Limite:
Animals
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
1983
Tipo de documento:
Article