Efficient extraction of RNA from mammalian tissue.
Mol Cell Biochem
; 56(2): 113-22, 1983.
Article
em En
| MEDLINE
| ID: mdl-6196612
ABSTRACT
RNA extraction from mammalian tissue has been compared using the different deproteinizing agents a) guanidine-HCl, b) guanidinium-thiocyanate, c) buffer-saturated phenol, or d) buffer-saturated phenol followed by a proteinase K digestion of the aqueous phase. Both solid tissues (first, second, and third trimester fetal bovine pancreas), and human white blood cell populations were studied. Degradation, as seen in citric acid-urea agarose gels, and the ability to serve as templates for cell-free protein synthesis were used as criteria to assess the efficiency of the different methods. We conclude that employing buffer-saturated phenol with proteinase K digestion is a superior method for consistent extraction of relatively undegraded RNA in quantitative amounts from mammalian tissue.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
RNA
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Mol Cell Biochem
Ano de publicação:
1983
Tipo de documento:
Article