A rabbit helper T cell clone reactive against group-specific streptococcal carbohydrate.
J Immunol
; 133(3): 1553-7, 1984 Sep.
Article
em En
| MEDLINE
| ID: mdl-6205087
ABSTRACT
Cloned rabbit T cell lines specific for the group-specific carbohydrate of group C streptococcus were established to study T cell responses to this antigen. T cell lines and clones of these lines were prepared from peripheral blood lymphoid cells of animals previously hyperimmunized with group C streptococcal vaccine. All cell lines studied were antigen specific in that they proliferated in the presence of group C but not group A vaccine. Three clones from one rabbit showed strong proliferation in the presence of soluble group C carbohydrate, in addition to the particulate vaccine. One of these clones, 5317.1, upon additional characterization, was shown to possess helper activity in that it induced autologous B cells to produce anti-carbohydrate antibody. Interestingly, B cells could not be stimulated by this carbohydrate-responsive clone to produce anti-carbohydrate antibody in the presence of soluble carbohydrate; the intact vaccine was required to demonstrate the helper effect of the clone. Phenotypic characterization of this clone with a panel of monoclonal antibodies indicated that it was of the T1 subpopulation of peripheral T cells. No suppressor activity was observed for any of the lines studied. Although the use of outbred animals in T cell cloning experiments has certain disadvantages, the present results indicate that cloned rabbit T cells may be useful tools for the elucidation of phenotypic and functional differences of rabbit T cell subsets.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polissacarídeos Bacterianos
/
Streptococcus
/
Linfócitos T Auxiliares-Indutores
/
Antígenos de Bactérias
Limite:
Animals
Idioma:
En
Revista:
J Immunol
Ano de publicação:
1984
Tipo de documento:
Article