Measurement of immunoglobulin A, G, and M class rotavirus antibodies in serum and mucosal secretions.
J Clin Microbiol
; 12(3): 314-9, 1980 Sep.
Article
em En
| MEDLINE
| ID: mdl-6260831
ABSTRACT
A solid-phase, enzyme-linked immunospecific assay for measurement of different immunoglobulin classes of human rotavirus antibodies is described. The antigen, which was adsorbed directly to polyvinyl microtiter plates, consisted of a clarified cell culture stock of the simian rotavirus SA 11. The assay was sensitive and reproducible and could readily be calibrated to determine concentrations of each class of antibody. The assay was applied to measurements of rotavirus antibodies in serum, colostrum, milk, and fecal samples. It particularly facilitates investigations of the role of immunoglobulin A antibodies in immunity to rotavirus infections.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Reoviridae
/
Imunoglobulina A
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Imunoglobulina G
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Imunoglobulina M
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Ensaio de Imunoadsorção Enzimática
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Técnicas Imunoenzimáticas
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Rotavirus
/
Anticorpos Antivirais
Limite:
Child, preschool
/
Female
/
Humans
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Infant
/
Pregnancy
Idioma:
En
Revista:
J Clin Microbiol
Ano de publicação:
1980
Tipo de documento:
Article