Nucleotide sequence of the simian virus 40 HindII + III restriction fragment A (second part of the T antigen gene).
Eur J Biochem
; 106(1): 169-77, 1980 May.
Article
em En
| MEDLINE
| ID: mdl-6280994
ABSTRACT
The nucleotide sequence of the second part of the simian virus 40 DNA HindII + III restriction fragment A is presented. The sequence extends from map position 0.533 to 0.424 and together with the first part of Hind-A [Volckaert et al. Proc. Natl Acad. Sci. U.S.A. 75, 2160--2164 (1978)] completes the total hind-A sequence, comprising 1169 base pairs. The second half of Hind-A includes the region corresponding to the second splicing boundary common to small tumor antigen (small-t) and large tumor antigen (large-T) mRNA and it contains coding information for an internal portion of large-T antigen. Two similar secondary structures of reasonable thermodynamic stability can be proposed for the nucleotide sequence of the pre-mRNA corresponding to the region reported here. Their possible relevance to the splicing of the SV40 early mRNAs is discussed. The deduced amino acid sequence is 188 residues long and contains a Lys-Lys-Lys-Arg-Lys-stretch which may be involved in the DNA binding capacity of large-T. A presumptive phosphorylation site is also present.
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Base de dados:
MEDLINE
Assunto principal:
DNA Viral
/
Desoxirribonucleases de Sítio Específico do Tipo II
/
Vírus 40 dos Símios
/
Genes Virais
/
Antígenos Virais
Idioma:
En
Revista:
Eur J Biochem
Ano de publicação:
1980
Tipo de documento:
Article