Production of purified hepatitis B surface antigen from Alexander hepatoma cells grown in artificial capillary units.
J Virol Methods
; 7(5-6): 263-71, 1983 Dec.
Article
em En
| MEDLINE
| ID: mdl-6330145
ABSTRACT
An artificial capillary system was devised for growth of hepatoma cells that yields very high titers of hepatitis B surface antigen (HBsAg). High yield of antigen was facilitated by slowing cellular metabolism through reduction of incubation temperature and addition of 0.1 mM caffeine. Deletion of serum from the medium did not reduce the yield of antigen. HBsAg prepared from the culture fluid by affinity chromatography and additional chemical and enzymatic steps was essentially pure and was indistinguishable from HBsAg prepared from infected human plasma. Preparation of HBsAg from the cell culture source presents advantages over that of human plasma and might be a source of HBsAg for vaccine preparation.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Carcinoma Hepatocelular
/
Técnicas de Cultura
/
Antígenos de Superfície da Hepatite B
/
Neoplasias Hepáticas
Limite:
Humans
Idioma:
En
Revista:
J Virol Methods
Ano de publicação:
1983
Tipo de documento:
Article