Simplified analytical methods for the measurement of the synthesis rate of plasma proteins in vivo by the [14C]carbonate method.
Ann Clin Biochem
; 21 ( Pt 5): 378-86, 1984 Sep.
Article
em En
| MEDLINE
| ID: mdl-6439102
ABSTRACT
We describe a method for obtaining the specific activity of 14C in urea, essential in the measurement of the synthesis rate of a plasma protein in vivo, which is simpler than the original procedure. The principle is the measurement of 14CO2 and NH4+ separately, after incubation with urease. A simple alteration gives samples of 13CO2 for mass spectrometry. The 'recoveries' of 14C and 13C in urea were invariably between 90 and 96% and the CV was 3%.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Carbonatos
/
Proteínas Sanguíneas
Limite:
Humans
Idioma:
En
Revista:
Ann Clin Biochem
Ano de publicação:
1984
Tipo de documento:
Article