Immunological and genetic characterization of 2-deoxygalactose-resistant, galactokinase-deficient mutants of Chinese hamster cells: evidence for structural mutations at the galK locus.
Mol Cell Biol
; 4(11): 2413-9, 1984 Nov.
Article
em En
| MEDLINE
| ID: mdl-6513922
ABSTRACT
Ten independent mutants resistant to 2-deoxygalactose and without any detectable galactokinase activity (null-galactokinase mutations) were isolated from mutagenized Chinese hamster somatic cells. They were analyzed for the presence of serologically cross-reacting material (CRM) with antiserum generated against highly purified Chinese hamster galactokinase. All 10 mutants contain cross-reacting material (i.e., were CRM+), indicating that all the mutations affect the correct expression of a product of the galactokinase structural gene. Complementation analysis among them shows that the 10 mutations fall in one functional genetic unit.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fucose
/
Galactoquinase
/
Mutação
Limite:
Animals
Idioma:
En
Revista:
Mol Cell Biol
Ano de publicação:
1984
Tipo de documento:
Article