The nucleotide sequence of cDNA complementary to the C1 component of rat prostatic binding protein.
Eur J Biochem
; 133(3): 645-9, 1983 Jul 01.
Article
em En
| MEDLINE
| ID: mdl-6688048
ABSTRACT
The mRNA for component C1 of rat prostatic binding protein has been cloned and characterized. A partially purified mRNA fraction for this complex protein was reverse-transcribed into double-stranded cDNA and cloned into the PstI site of plasmid pBR 322. The 426-base-pair insert of the recombinant plasmid pC1A75 was completely sequenced. The coding region corresponds precisely to the 88 amino acid residues of C1 and in addition contains the information of a signal peptide of 23 residues. The 5' non-coding region counts only 19 nucleotides and is incomplete but the 3'-terminal non-coding part of 60 nucleotides extends into the poly(A) tail. Sequence analysis of other C1-positive clones indicates the presence of sequence rearrangements which must have occurred during the cloning procedure. Possible mechanisms for the generation of these cloning artefacts are discussed.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fragmentos de Peptídeos
/
Próstata
/
DNA
/
RNA Mensageiro
/
Proteínas de Transporte
/
Proteína de Ligação a Androgênios
Limite:
Animals
Idioma:
En
Revista:
Eur J Biochem
Ano de publicação:
1983
Tipo de documento:
Article