Fatty acid desaturase specificity of Tetrahymena.
Lipids
; 19(4): 285-8, 1984 Apr.
Article
em En
| MEDLINE
| ID: mdl-6717257
ABSTRACT
The ciliate, Tetrahymena, was provided a supplement of the fatty acid [1-14 C]182 delta 6.9. After a period of growth the cells were claimed, the lipids extracted, the polar lipids recovered and the mild alkali-labile fatty acid methyl esters generated. The fatty acids were resolved by high pressure liquid chromatography (HPLC), the 183 delta 6.9,12(gamma-linolenic acid) was recovered and its identity verified by high pressure liquid chromatography (HPLC), gas liquid chromatography (GLC), hydrogenation and oxidation. Fifty-three percent of the cell-associated label was found in gamma-linolenic acid; thus, a delta 12 fatty acid desaturase converts the 6,9 octadecadienoic acid to the 6,9,12 derivative. No carboxyl or methyl terminus restriction appears on delta 9 monoenoic or dienoic fatty acid desaturation in this cell as is found in higher plants and animals.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Tetrahymena
/
Ácidos Graxos Dessaturases
Idioma:
En
Revista:
Lipids
Ano de publicação:
1984
Tipo de documento:
Article