A fluorescein-linked immunoabsorbent assay for the detection of antibacterial antibodies in secretions and serum.
J Immunol Methods
; 53(3): 335-43, 1982 Sep 30.
Article
em En
| MEDLINE
| ID: mdl-6754816
ABSTRACT
A rapid, quantitative solid-phase immunofluorescence assay has been developed to measure antibodies reactive with Streptococcus mutans in saliva and serum. Formalin killed bacteria were adsorbed to cellulose acetate discs and antibodies bound to the antigen-coated immunoabsorbent were detected by use of fluorescein-labeled antibody to human immunoglobulin isotypes. Quantitation was performed by placing the immunoabsorbent discs in a fluorometer. Low levels of naturally occurring antibodies reactive with S. mutans were easily detected in saliva and serum from normal human subjects.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fluoresceínas
/
Anticorpos Antibacterianos
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
J Immunol Methods
Ano de publicação:
1982
Tipo de documento:
Article