Specific photoisomerization of retinal in squid rhodopsin and metarhodopsin.
Biochim Biophys Acta
; 636(1): 27-31, 1981 Jun 12.
Article
em En
| MEDLINE
| ID: mdl-7284342
ABSTRACT
The composition of retinal isomers in the photosteady-state mixtures formed from squid rhodopsin and metarhodopsin was determined by high-pressure liquid chromatography. A large amount of 9-cis-retinal was obtained at liquid N2 temperature when rhodopsin was irradiated with orange light, but only small quantities of 9-cis-retinal were obtained at 15 degrees C. Scarcely any 9-cis-retinal was produced from metarhodopsin by irradiation at liquid N2 temperature. A large quantity of 7-cis-retinal was found in the photoproduct of rhodopsin irradiated at solid carbon dioxide temperature, but not at 15 degrees C and liquid N2 temperature. 7-cis-Retinal was not produced from metarhodopsin at any temperatures. These results indicate that the photoisomerization of retinal is regulated by the structure of the retinal-binding site of this protein. The formation of 9-cis- and 7-cis-retinals is forbidden in the metarhodopsin protein.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pigmentos da Retina
/
Retinaldeído
/
Rodopsina
/
Decapodiformes
/
Vitamina A
/
Luz
Limite:
Animals
Idioma:
En
Revista:
Biochim Biophys Acta
Ano de publicação:
1981
Tipo de documento:
Article