Three-dimensional mammalian cell growth on nonwoven polyester fabric disks.
Biotechnol Prog
; 10(5): 548-50, 1994.
Article
em En
| MEDLINE
| ID: mdl-7522468
ABSTRACT
Small disks of nonwoven polyester fabric (NWPF), similar to Fibra-Cel carriers, were surface treated with strong acid and used as a high surface area support matrix for in vitro culture of anchorage dependent MRC-5 cells. The disks can be autoclaved, and require inocula concentrations of only 2-5% of the final cell densities, which have reached 10(8) cells/mL of disk bed volume. Scanning electron microscopy photographs shown herein reveal that cells grow in a multilayered fashion between the randomly arrayed fibers of the nonwoven fabric, emulating in vivo growth.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Poliésteres
/
Divisão Celular
/
Fibroblastos
Limite:
Humans
Idioma:
En
Revista:
Biotechnol Prog
Assunto da revista:
BIOTECNOLOGIA
Ano de publicação:
1994
Tipo de documento:
Article