Tetrameric structure and cellular location of catechol 2,3-dioxygenase.
Arch Microbiol
; 163(1): 65-9, 1995 Jan.
Article
em En
| MEDLINE
| ID: mdl-7710322
Catechol 2,3-dioxygenase from the meta-cleavage pathway encoded on the TOL plasmid of Pseudomonas putida (pWWO) was investigated by electron microscopy. Negatively stained samples of the purified catechol 2,3-dioxygenase revealed that the enzyme consists of four subunits arranged in a tetrahedral conformation. Monoclonal antibodies raised against catechol 2,3-dioxygenase showed highly specific reactions and were used to localize the enzyme in Escherichia coli (pAW31) and P. putida (pWWO), using the protein A-gold technique carried out as a post-embedding immunoelectron microscopy procedure. Our in situ labeling studies revealed a cytoplasmic location of the catechol 2,3-dioxygenase in both cell types.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oxigenases
/
Conformação Proteica
/
Pseudomonas putida
/
Dioxigenases
/
Escherichia coli
Idioma:
En
Revista:
Arch Microbiol
Ano de publicação:
1995
Tipo de documento:
Article
País de afiliação:
Alemanha