On-line immunoanalysis for bioprocess control.
J Biotechnol
; 31(3): 395-403, 1993 Dec.
Article
em En
| MEDLINE
| ID: mdl-7764446
ABSTRACT
Immunoanalytical techniques such as ELISA are often used for the detection of proteins produced in cultivation processes. Owing to the difficulty of automating of the time-consuming traditional ELISA, there is an intense demand for a suitable on-line monitoring method. Combining well-known immunoassays with the FIA technique, we present the heterogeneous and the turbidimetric immuno-FIA methods. The following proteins were investigated with these FIA methods:
thermostable pullulanase, IgG, antithrombin III, and recombinant tissue-type plasminogen activator. In the cases of pullulanase and monoclonal mouse IgG, the turbidimetric immuno-FIA was used for on-line analysis of the cultivation process. Results are presented here to demonstrate the effectiveness and application of these immunoanalysis.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Imunoglobulina G
/
Técnicas Biossensoriais
/
Antitrombina III
/
Técnicas Imunoenzimáticas
/
Ativador de Plasminogênio Tecidual
/
Glicosídeo Hidrolases
Idioma:
En
Revista:
J Biotechnol
Assunto da revista:
BIOTECNOLOGIA
Ano de publicação:
1993
Tipo de documento:
Article
País de afiliação:
Alemanha