Description of a 700-kb yeast artificial chromosome contig containing the BCL1 translocation breakpoint region at 11q13.
Cytogenet Cell Genet
; 69(1-2): 101-7, 1995.
Article
em En
| MEDLINE
| ID: mdl-7835075
ABSTRACT
We screened two human yeast artificial chromosome (YAC) libraries by polymerase chain reaction (PCR) with oligonucleotides specific to the BCL1 major translocation breakpoint cluster region at 11q13. Five YACs were isolated. Two of them were chimeric. One of these and remaining three YACs were characterized by hybridization with various known 11q13 probes, Alu-PCR fingerprinting, in situ hybridization, and isolation of YAC ends. A map of this ca 700-kb YAC contig was obtained. This map was consistent with maps established from total human genomic DNA. Every YAC in this region was found unstable and gave rise to reproducibly deleted lineages. Analysis in detail of these deletions over many generations showed that more than a single sequence might be involved. The availability of cloned material will facilitate the search for the still elusive genetic elements responsible for amplifications, deletions and translocations observed at 11q13 in malignancies.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Translocação Genética
/
Cromossomos Humanos Par 11
/
Proto-Oncogenes
/
Proteínas Proto-Oncogênicas
/
Cromossomos Artificiais de Levedura
Limite:
Humans
Idioma:
En
Revista:
Cytogenet Cell Genet
Ano de publicação:
1995
Tipo de documento:
Article
País de afiliação:
França