Cell cycle kinetic effects of tamoxifen on human breast cancer cells. Flow cytometric analyses of DNA content, BrdU labeling, Ki-67, PCNA, and statin expression.
Ann N Y Acad Sci
; 698: 174-81, 1993 Nov 30.
Article
em En
| MEDLINE
| ID: mdl-7904138
ABSTRACT
Tamoxifen is known to inhibit the growth of some human mammary carcinoma cells; this effect is accompanied by a decrease in the proportion of cells synthesizing DNA. In this work, flow cytometry of DNA and of bromodeoxyuridine labeling and the evaluation of the cell cycle-related antigens Ki-67, PCNA, and statin were used to investigate the changes in the proliferation kinetics of MCF-7 cells before and after treatment with 10(-7) M TAM. The treatment with TAM induced a significant decrease in the fraction of S-phase cells and an increase in those with a DNA content typical of G0/1 phase. The TAM-induced block in G0/1 is paralleled by a decrease in the frequency of cells expressing Ki-67 and PCNA, and by an increase in statin-positive (G0) cells. These results confirmed that the TAM-induced inhibition of cell growth is associated with major changes in the cell cycle parameters of MCF-7 cells, and provide the first experimental evidence that two main mechanisms are operating the accumulation of cells in G1, before the onset of S-phase, and the exit of some cells from the cycling compartment.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Tamoxifeno
/
Neoplasias da Mama
/
DNA de Neoplasias
/
Proteínas Nucleares
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Proteínas
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Biomarcadores Tumorais
/
Ciclo Celular
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Proteínas de Neoplasias
Limite:
Female
/
Humans
Idioma:
En
Revista:
Ann N Y Acad Sci
Ano de publicação:
1993
Tipo de documento:
Article
País de afiliação:
Itália