Study on the surface polypeptides isolated from human lymphoblastoid ce-ls by ionic shock.
Biochim Biophys Acta
; 444(3): 863-74, 1976 Oct 22.
Article
em En
| MEDLINE
| ID: mdl-791366
ABSTRACT
Ionic shock treatment in the presence of 10% glycerol is an efficient and selective method for extracting cell surface components from Raji cells and effecting the solubilization of up to 22 polypeptides. The majority of these shock-released polypeptides are accessible to lactoperoxidase radioiodination. Sera from rabbits immunized against these soluble extracts are reactive against Raji cell surface as indicated by indirect membrane immunofluorescence and agglutination assays.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Linfócitos
Limite:
Humans
Idioma:
En
Revista:
Biochim Biophys Acta
Ano de publicação:
1976
Tipo de documento:
Article