Development of a live, oral, attenuated vaccine against El Tor cholera.
J Infect Dis
; 170(6): 1518-23, 1994 Dec.
Article
em En
| MEDLINE
| ID: mdl-7995992
Vibrio cholerae El Tor strains from Peru, Bangladesh, and Bahrain were attenuated by deletion of a genetic element that encodes virulence factors and RS1. The B subunit of ctx (ctxB) was reintroduced into the recA gene of the deletion mutants, rendering them unable to recombine with exogenous genetic elements and generating Peru-3, Bang-3, and Bah-3. Fifteen volunteers received one dose of various vaccine strains at 4 x 10(6) to 1 x 10(8) cfu. All strains colonized the gut. A > or = 4-fold rise in vibriocidal titer was observed in 14 volunteers, with titers of > or = 1600 in 13. Peru-3 was the least reactogenic, but 2 of 6 volunteers had loose stools. Peru-14, a filamentous motility-deficient mutant of Peru-3, was well tolerated and colonized 18 of 21 volunteers at doses of 2 x 10(6) to 1 x 10(9) cfu. Also, when 8 Peru-3 or Peru-5 vaccinees, 5 Peru-14 vaccinees, and 8 controls were challenged with 2 x 10(6) cfu V. cholerae El Tor Inaba (N16961), 11 vaccinees were protected compared with no controls. Peru-14 shows promise as a safe, effective, single-dose oral vaccine against El Tor cholera.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vacinas contra Cólera
/
Cólera
Tipo de estudo:
Clinical_trials
Limite:
Adolescent
/
Adult
/
Animals
/
Female
/
Humans
/
Male
Idioma:
En
Revista:
J Infect Dis
Ano de publicação:
1994
Tipo de documento:
Article