A new assay based on thrombin generation inhibition to detect both protein C and protein S deficiencies in plasma.
Thromb Haemost
; 71(3): 331-8, 1994 Mar.
Article
em En
| MEDLINE
| ID: mdl-8029798
ABSTRACT
Activated protein C reduces thrombin generation by inactivating factors V and VIII in the presence of protein S. This prompted us to develop an assay which would allow specific exploration of this reaction. The total amount of thrombin formed in plasma after activation by tissue factor and phospholipids was reduced by adding thrombomodulin. This addition allowed protein C to be activated by endogenous thrombin. The inhibition of thrombin generation (ITG) due to protein C activation could be measured by comparing thrombin formation in the presence and in the absence of thrombomodulin. ITG increased with both protein C and protein S concentrations. Normal values of ITG expressed as a percentage were between 40 and 65% and were not influenced by age or sex. ITG increased in patients under heparin therapy, decreased in patients under oral anticoagulant therapy and was decreased in women using oral contraceptives. This method could be used for screening patients for protein C and protein S deficiencies.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Testes de Coagulação Sanguínea
/
Trombina
/
Deficiência de Proteína S
/
Deficiência de Proteína C
Limite:
Animals
/
Female
/
Humans
Idioma:
En
Revista:
Thromb Haemost
Ano de publicação:
1994
Tipo de documento:
Article
País de afiliação:
França