GC-MS determination of ratios of stable-isotope labelled to natural urea using [13C15N2]urea for studying urea kinetics in serum and as a means to validate routine methods for the quantitative assay of urea in dialysate.
Clin Chim Acta
; 225(1): 29-42, 1994 Feb.
Article
em En
| MEDLINE
| ID: mdl-8033352
ABSTRACT
A GC-MS determination of urea in serum or spent dialysate is described, using 13C15N2-labelled urea and assaying the area ratio of labelled to natural urea by mass fragmentographic monitoring of fragments m/e 153 and 156, after its eventual conversion into the trimethylsilylether-derivative of 2-hydroxypyrimidine. The procedure can be successfully applied in the follow-up of the disappearance of labelled urea in serum after intravenous injection in man, enabling kinetic parameters of urea to be established, e.g. for purposes of studying the effectiveness of dialysis procedures. Furthermore the method can be used for validation of routine methods for measuring urea in other fluids, in particular dialysate. Examples are given of both applications of the GC-MS method described.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ureia
Limite:
Humans
/
Male
/
Middle aged
Idioma:
En
Revista:
Clin Chim Acta
Ano de publicação:
1994
Tipo de documento:
Article
País de afiliação:
Holanda