Characterisation of angiotensin II binding to glomeruli from rainbow trout Oncorhynchus mykiss adapted to fresh water and seawater.
Gen Comp Endocrinol
; 94(1): 104-12, 1994 Apr.
Article
em En
| MEDLINE
| ID: mdl-8045359
ABSTRACT
Specific binding of 125I-[Asn1, Val5]-angiotensin II to isolated glomeruli of freshwater-adapted and seawater-adapted rainbow trout, Oncorhynchus mykiss, reached a plateau after 20 min at 10 degrees and was displaced by nonradiolabelled [Asn1, Val5]-angiotensin II. Seawater adaptation increased the rate of dissociation of 125I-[Asn1, Val5]-angiotensin II from putative receptors. Competitive binding experiments revealed a single population of high-affinity receptors with a KD 0.3-0.4 x 10(-10) M, which was unchanged by seawater adaptation. Seawater adaptation did, however, reduce the density (Bmax) of the binding sites, which may reflect down-regulation by the higher plasma concentration of angiotensin II in seawater-adapted trout.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Água do Mar
/
Angiotensina II
/
Adaptação Fisiológica
/
Oncorhynchus mykiss
/
Água Doce
/
Glomérulos Renais
Limite:
Animals
Idioma:
En
Revista:
Gen Comp Endocrinol
Ano de publicação:
1994
Tipo de documento:
Article
País de afiliação:
Reino Unido