Identification of a Xenopus cDNA that prevents mitotic catastrophe in the fission yeast Schizosaccharomyces pombe.
Gene
; 145(1): 155-6, 1994 Jul 22.
Article
em En
| MEDLINE
| ID: mdl-8045419
ABSTRACT
A Xenopus total ovary cDNA library was constructed in a fission yeast expression vector. Using a genetic functional complementation method, we have identified a Xenopus cDNA clone that can rescue several different yeast mitotic catastrophe mutants defective in Wee1 kinase function at the restrictive temperature. The 3.0-kb cDNA clone contains an open reading frame (ORF) of 2226 nucleotides, encoding a predicted 82-kDa protein. The deduced amino acid (aa) sequence shows seven almost identical 30-aa tandem repeats, each of which contains a phosphorylation site meeting the consensus for both Cdc2 kinase and MAP kinase.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Schizosaccharomyces
/
Proteínas Tirosina Quinases
/
Proteínas Nucleares
/
Proteínas de Ciclo Celular
/
Proteínas de Xenopus
/
Mitose
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
Gene
Ano de publicação:
1994
Tipo de documento:
Article