Voltage-dependent Ca2+ channels in Chinese hamster ovary (CHO) cells.
FEBS Lett
; 349(2): 289-94, 1994 Aug 01.
Article
em En
| MEDLINE
| ID: mdl-8050584
ABSTRACT
Voltage-dependent Ca2+ channels were identified in CHO-K1 cells, currently used in molecular biology studies. Experimental data obtained at both macroscopic and single-channel levels using the patch-clamp technique show that the Ca2+ current in CHO cells is similar to the high-threshold L-type of Ca2+ current previously observed in excitable cells. It can be carried by Ca2+ or Ba2+ ions, blocked by both inorganic (Co2+) and organic (nifedipine, isradipine) Ca2+ channel blockers. The unitary Ca2+ channel activity was characterized by a conductance of 19 pS in 60 mM Ca2+, by single exponential distribution of open times (t = 0.84 ms) and biexponential distribution of closed times (tf = 1.67 ms, ts = 7.9 ms). However, the functional role of these Ca2+ channels in CHO cells remains unclear.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Canais de Cálcio
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
1994
Tipo de documento:
Article
País de afiliação:
França