Ultraviolet absorbance and circular dichroism of Pf1 virus: nucleotide/subunit ratio of unity, hyperchromic tyrosines and DNA bases, and high helicity in the subunits.
Biochemistry
; 33(7): 1694-703, 1994 Feb 22.
Article
em En
| MEDLINE
| ID: mdl-8110771
ABSTRACT
Data have been obtained for the Pf1 virion that establish its stoichiometry and conformational features of its DNA and its protein. The absorbance spectrum of the dissociated virus under alkaline denaturing conditions is fit exactly by spectra for DNA and protein at a mole ratio of one nucleotide per protein subunit. This result, together with three previous values by independent methods, establishes that the nucleotide/subunit ratio (n/s) of Pf1 is unity. The absorbance spectrum of DNA in the intact native virus is assigned as the spectrum for heat denatured Pf1 DNA, with epsilon (P) = 8400 M-1 cm-1 at 259 nm. The absorbance spectrum assigned to protein (two tyrosines) in the intact virus has
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01-internacional
Base de dados:
MEDLINE
Assunto principal:
Tirosina
/
Proteínas Virais
/
DNA Viral
/
Inovirus
Idioma:
En
Revista:
Biochemistry
Ano de publicação:
1994
Tipo de documento:
Article