Morphologic and metabolic changes in rat osteoblast cultures during the dark reaction with 8-methoxypsoralen.
Chem Biol Interact
; 90(2): 185-93, 1994 Feb.
Article
em En
| MEDLINE
| ID: mdl-8156607
ABSTRACT
The dark reaction of 8-methoxypsoralen (8-MOP) with cultured rat osteoblasts did not cause significant changes in cellular replication rates or in the synthesis of RNA and proteins. Microscopic examination, however, revealed that the dark reaction resulted in massive accumulation of perinuclear lipids and in the statistically significant enhancement of alkaline phosphatase activity. A sharp, and statistically significant, upsurge of lipid synthesis in osteoblasts preceded microscopically detectable accumulation of lipids and occurred only during the initial, but not during the subsequent stages of the dark reaction. These results suggest that in the course of the dark reaction the plasma membrane of osteoblasts is a target of psoralen.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Osteoblastos
/
Metoxaleno
Limite:
Animals
Idioma:
En
Revista:
Chem Biol Interact
Ano de publicação:
1994
Tipo de documento:
Article