Plasmid DNA is superior to viral vectors for direct gene transfer into adult mouse skeletal muscle.
Hum Gene Ther
; 4(6): 733-40, 1993 Dec.
Article
em En
| MEDLINE
| ID: mdl-8186288
ABSTRACT
Direct gene transfer into skeletal muscle offers several therapeutic possibilities. We assessed direct intramuscular injection of recombinant plasmids, adenovirus, or retrovirus in normal or regenerating muscles of mice. The incorporation and expression of reporter genes introduced by any of these three vectors is greater in regenerating than in mature muscle. In regenerating muscle, pure DNA and adenovirus result in equivalent numbers of fibers expressing reporter gene (> 10%), but adenovirus also induces considerable cellular infiltration. In mature muscle, recombinant DNA is better than adenovirus. Retrovirus failed to infect mature muscle fibers and was less effective than plasmid DNA or adenovirus in regenerating muscle. The surprisingly high relative efficiency of pure plasmid DNA suggests that this method will provide a simple, safe and viable alternative for gene therapy involving muscle tissue.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plasmídeos
/
DNA Recombinante
/
DNA Viral
/
Técnicas de Transferência de Genes
/
Vetores Genéticos
/
Músculos
Limite:
Animals
Idioma:
En
Revista:
Hum Gene Ther
Assunto da revista:
GENETICA MEDICA
/
TERAPEUTICA
Ano de publicação:
1993
Tipo de documento:
Article
País de afiliação:
Canadá