A pH induced two-dimensional crystal of membrane-bound Na+,K(+)-ATPase of dog kidney.
FEBS Lett
; 320(1): 17-22, 1993 Mar 29.
Article
em En
| MEDLINE
| ID: mdl-8385024
ABSTRACT
Two-dimensional crystals of membrane-bound Na+,K(+)-ATPase were formed in acidic media and their qualities were investigated by electron cryo-microscopy as well as by conventional electron microscopy. At pH 4.8 in sodium citrate buffer, the best crystallization condition, more than 80% of membranes formed crystals. The high ratio allowed high-resolution images to be taken by electron cryo-microscopy. Image processing revealed that they had unique lattice constants (a = 108.7 A, b = 66.2 A, gamma = 104.2 degrees) and had few defects in the crystalline arrays. The reconstituted Fourier map of the ice-embedded crystal showed that there are two high contrast parts in one unit cell.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
ATPase Trocadora de Sódio-Potássio
/
Rim
Limite:
Animals
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
1993
Tipo de documento:
Article
País de afiliação:
Japão