Analysis of T cell receptor variability in tumor-infiltrating lymphocytes from a human regressive melanoma. Evidence for in situ T cell clonal expansion.
J Clin Invest
; 91(3): 1183-90, 1993 Mar.
Article
em En
| MEDLINE
| ID: mdl-8450047
ABSTRACT
Malignant melanomas are often infiltrated by T lymphocytes. It is postulated that the presence of tumor-infiltrating lymphocytes (TIL) reflects ongoing immune responses against transformed cells. Such "responses" appear generally inefficient with the potential exception of infrequent clinical situations characterized by spontaneous tumor regression. We have characterized here the molecular structure of the T cell receptor beta chain expressed by TILs in a case of regressive melanoma. Advantage was taken of the PCR technology to study T lymphocytes directly without cell culture. Experimentally validated V beta subfamily specific primers were used to evaluate the V beta usage in TILs and control samples. Our results reveal that clonal T cell populations, precisely defined by their V-D-J junctional sequences, are amplified at the tumor site. The existence of such local antigen-driven selections support the hypothesis that antitumor responses may indeed take place in regressive melanoma.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neoplasias Cutâneas
/
Variação Genética
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Receptores de Antígenos de Linfócitos T
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Linfócitos T
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Linfócitos do Interstício Tumoral
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Melanoma
Limite:
Aged
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Female
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Humans
Idioma:
En
Revista:
J Clin Invest
Ano de publicação:
1993
Tipo de documento:
Article
País de afiliação:
França