[Phencyclidine receptors in porcine coronary artery].
Zhongguo Yao Li Xue Bao
; 14(1): 39-41, 1993 Jan.
Article
em Zh
| MEDLINE
| ID: mdl-8503285
ABSTRACT
By using radioligand assay, there was a phencyclidine (Phe) binding site in porcine coronary artery. This binding was specific, reversible, saturable, and stereoselective. The Scatchard analysis showed that the binding site was a single class, with dissociation constant (Kd) and maximum binding (Bmax) of 27.7 +/- 6.9 nmol.L-1 and 0.82 +/- 0.15 pmol/mg protein, respectively. The displacement experiments revealed that the [3H]Phe binding was displaced by nonradioactive Phe, TCP (Phe receptor agonists), and dextrophan (its antagonist). d-INN, a ligand of sigma receptor, had a weaker activity of displacement. These showed a dose-dependent manner. Both etorphine, an agonist of opioid receptor, and N-methyl-D-aspartic acid (NMDA), an excitatory amino acid, failed to displace the binding. These results suggest that the Phe receptors exist in the porcine coronary artery.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Receptores da Fenciclidina
/
Vasos Coronários
Limite:
Animals
Idioma:
Zh
Revista:
Zhongguo Yao Li Xue Bao
Ano de publicação:
1993
Tipo de documento:
Article
País de afiliação:
China