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Cultured pig rhabdomyosarcoma cells with a deletion of the Xq24-qter chromosome region: an immunochemical and cytogenetic characterization.
van der Loop, F T; Bosma, A A; Vos, J H; Mirck, M H; Schaart, G; van den Ingh, T S; Ramaekers, F C.
Afiliação
  • van der Loop FT; Department of Molecular Cell Biology and Genetics, University of Limburg, Maastricht, The Netherlands.
Am J Vet Res ; 56(8): 1062-9, 1995 Aug.
Article em En | MEDLINE | ID: mdl-8533978
A pig rhabdomyosarcoma cell line (PRUM59) was established, and the immuno(histo)chemical and cytogenetic characterization of these cells was determined. At various swine farms in the Netherlands, pigs were observed that had solitary or multiple skin nodules, which were diagnosed as rhabdomyosarcomas. Cells of a tumor derived from a 3.5-week-old female pig were cultured for immunochemical and cytogenetic analyses. The cell line had characteristic features of undifferentiated muscle cells, similar to those observed in tumor tissue sections; they contained titin, a high-molecular weight protein specific for striated muscle, as dot-like aggregates and as filaments, desmin filaments and cross-striations, smooth muscle actin stress fibers, and vimentin filaments. The cells stained positively for striated muscle actin and tropomyosin as well. The immunohistochemical staining results were supported by results of immunoblotting experiments. Karyotyping of the cells revealed a deletion of a major part of Xq24-qter, a part of the long arm of 1 of the 2 X chromosomes. The other X chromosome and all autosomes appeared to be normal.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Rabdomiossarcoma / Doenças dos Suínos / Cromossomo X / Deleção Cromossômica Limite: Animals Idioma: En Revista: Am J Vet Res Ano de publicação: 1995 Tipo de documento: Article País de afiliação: Holanda
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Rabdomiossarcoma / Doenças dos Suínos / Cromossomo X / Deleção Cromossômica Limite: Animals Idioma: En Revista: Am J Vet Res Ano de publicação: 1995 Tipo de documento: Article País de afiliação: Holanda