[Determination of amikacin by single oscillopolarography in the presence of formaldehyde].

ABSTRACT

The singlescan oscillopolarographic behavior of formaldehyde derivative of amikacin (AMK) in the medium of Britton-Robinson buffer solution (pH 8.0) has been studied and the mechanism of its electrode process has been discussed. Under optimum conditions, the peak current increases linearly with the increase of AMK concentration in the range of 6.0 x 10(-7)-5.0 x 10(-5) mol.L-1 and its correlation coefficient is 0.9988. The detection limit is 2.4 x 10(-7) mol.L-1. This method has been applied to the determination of trace AMK in samples of its injection, urine and blood serum without pretreatment. Its recovery was 90%-100% and the relative standard deviation was less than 4.1%.