A non-expressed transgene as a cell marker for the investigation of cellular traffic after splenic autotransplantation.
J Immunol Methods
; 194(2): 131-9, 1996 Aug 14.
Article
em En
| MEDLINE
| ID: mdl-8765166
ABSTRACT
The investigation of cellular interactions during the regeneration of splenic transplants depends upon distinguishing host cells from graft derived cells. As a cell marker which, in contrast to many other marker systems, does not affect histocompatibility, the non-expressed transgene mMT-HGHRH-1 was introduced by mating into NMRI inbred mice to generate the transgenic mouse line BSM. By transplanting non-transgenic NMRI splenic tissue into transgenic BSM hosts, host derived cells could be identified in the transplants by in situ hybridization after regeneration. In a reciprocal approach, implant derived cells were detected in a transgenic transplant after 3 weeks of regeneration in a non-transgenic host. Relative amounts of transgenic cells in transplant cross-sections were estimated from the signal intensity of autoradiographs by densitometry and computer analysis. This approach could be broadly applied in studies of transplantation systems.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Regeneração
/
Baço
/
Transgenes
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
J Immunol Methods
Ano de publicação:
1996
Tipo de documento:
Article
País de afiliação:
Áustria