Fatty acids selectively inhibit eukaryotic DNA polymerase activities in vitro.

Mizushina, Y; Tanaka, N; Yagi, H; Kurosawa, T; Onoue, M; Seto, H; Horie, T; Aoyagi, N; Yamaoka, M; Matsukage, A; Yoshida, S; Sakaguchi, K

Mizushina, Y; Tanaka, N; Yagi, H; Kurosawa, T; Onoue, M; Seto, H; Horie, T; Aoyagi, N; Yamaoka, M; Matsukage, A; Yoshida, S; Sakaguchi, K.

Afiliação

Mizushina Y; Department of Applied Biological Science, Faculty of Science and Technology, Science University of Tokyo, Chiba-ken, Japan.

Biochim Biophys Acta ; 1308(3): 256-62, 1996 Sep 11.

Article em En | MEDLINE | ID: mdl-8809118

ABSTRACT

ABSTRACT

The in vitro relationship between eukaryotic DNA polymerases and fatty acids was investigated. Some fatty acids strongly inhibited the activities of DNA polymerase alpha and/or beta in vitro. The kinetics of inhibition by linoleic acid showed that DNA polymerase alpha was non-competitively inhibited with respect to the DNA template and substrate (dTTP), while DNA polymerase beta was inhibited competitively with both DNA and substrate.

Assuntos

Ácidos Graxos/farmacologia; Inibidores da Síntese de Ácido Nucleico; Animais; Bovinos; DNA Polimerase I/antagonistas & inibidores; DNA Polimerase I/metabolismo; DNA Polimerase II/antagonistas & inibidores; DNA Polimerase II/metabolismo; DNA Polimerase Dirigida por DNA/metabolismo; Detergentes/farmacologia; Ácidos Graxos/química; Cinética; Ácido Linoleico; Ácidos Linoleicos/farmacologia; Octoxinol; Polietilenoglicóis/farmacologia; Ratos; Nucleotídeos de Timina/metabolismo

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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Inibidores da Síntese de Ácido Nucleico / Ácidos Graxos Limite: Animals Idioma: En Revista: Biochim Biophys Acta Ano de publicação: 1996 Tipo de documento: Article País de afiliação: Japão

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