Identification of the Leishmania infantum P0 ribosomal protein epitope in canine visceral leishmaniasis.
Immunol Lett
; 48(1): 23-8, 1995 Nov.
Article
em En
| MEDLINE
| ID: mdl-8847086
ABSTRACT
In the present work we show that a high percentage of the sera from dogs naturally affected with viscero-cutaneous leishmaniasis contain antibodies reacting with the Leishmania infantum P0 ribosomal protein. In order to map the antigenic determinants of the LiP0 protein during Leishmania-infection, the complete amino acid sequence of the protein was synthesized as overlapping 20-mer peptides. We have identified the sequence AAKEEPEESDEDDFGMG, located adjacent to the C-terminal end of the protein, as the major antigenic determinant. The anti-LiP0 antibodies present in the sera of the infected dogs do not cross-react with a relatively similar antigenic determinant of the LiP2 acidic proteins as an indication that the Leishmania P0 protein is an independent immunogenically functional antigen in the canine form of the infection.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Ribossômicas
/
Proteínas de Protozoários
/
Leishmania infantum
/
Leishmaniose Visceral
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
Immunol Lett
Ano de publicação:
1995
Tipo de documento:
Article
País de afiliação:
Espanha